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PCR at high altitude

David Watson dwatson at PLAINS.NODAK.EDU
Thu Mar 23 17:36:08 EST 1995

Dear Netters:

A quick observation (somebody correct me if this is not accurate):

Taq is an enzyme, albeit one with a very high temperature optimum.
Therefore, at a temp slightly below boiling in Quito (89 C, was it?),
shouldn't Taq still work, even if slightly less than optimally, such that
routine amplifications could still be accomplished? Does anyone know how
rapidly Taq's efficiency falls off either side of 94 C? I assume the dropoff
is steeper above 94 C?
  David A. Watson, Ph.D. (dwatson at plains.nodak.edu)  
  Asst. Prof. of Veterinary and Microbiological Sciences       
  North Dakota State University 
                   "Some are wise, some otherwise."                           

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