Peter Thoren wrote:
>hi netters!
>I am going to test spermidine in order to improve my microsatellite PCR.
>I just want to know what final concentration is used by others.
>Thanks in advance,
>Peter Thoren
>Dept.of genetics, Uppsala University
>Sweden
>peter.thoren at genetik.uu.se
The optimal range cited in a paper on this topic (Ching-Yi Wan and Thea A.
Wilkins (1993) Spermidine facilitates PCR amplification of target DNA. PCR
Methods and Applications 3: 208-210) is between 0.2 to 1.0 mM.
Regards - Juerg
Juerg E. Frey
Swiss Federal Research Station
for Fruit-Growing, Viticulture and Horticulture
CH-8820 Waedenswil
SWITZERLAND
PH: xxx411-783-6111
FAX:xxx411-780-6341
e-mail: juerg.frey at wae.faw.admin.ch
