Finally the solution to our PCR problem. It has been a long time
since we received the last suggestions for this problem and we hope that
you can still remember our questions about PCR at 3050 meters above sea
level. Water boils at 89 C and we were afraid that we would not be able
to heat up the tubes to 94 C.
We are very grateful to all of you who thought about it and gave
us suggestions. Fortunately, the capped tubes consisting sample and
mineral oil did not pop open and reached 94 C without problem. So far we
have not been able to find out if the high altitude causes other problems.
With friendly greetings,
Greg, Andre and Mariu.
CIP-Quito.
--
Andre van 't Slot
Centro Internacional de la Papa
Casilla 17-16-129-CEQ
Quito, Ecuador
Telf. +593-2-690990, Internet: VANTSLOT at cip.org.ec
--
Andre van 't Slot
Centro Internacional de la Papa
Casilla 17-16-129-CEQ
Quito, Ecuador
Telf. +593-2-690990, Internet: VANTSLOT at cip.org.ec