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now you see it now you don't

Kevin O'Donnell odonnell at sasa.gov.uk
Wed Nov 1 09:29:27 EST 1995

Rob Harling wrote:
> PCR works if we use freshly - prepared DNA, ie extracted from the 
> bacteria then run on the same day.  If we store the DNA at 4C for 
> only one week, then PCR fails, we get zilch in the lanes at any 
> concentration of template.  The DNA still looks the same after one week
> when run on a gel, ie there doesn't seem to be nuclease breakdown
> of the DNA.  Does anyone know what's happening to the DNA in storage?

Do you know if it still cuts with restriction endonucleases?


Dr Kevin O'Donnell		     "Work as if you were in the early days
Diagnostics and Molecular Biology         of a better nation"  Alasdair Gray
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell at sasa.gov.uk          

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