> Date: Sun, 05 Nov 95 01:47:35
> From: <pohyam at technet.sg>
> Subject: precipitation problems
> To: mx%"diagnost at net.bio.net"
> Cc:
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> To: diagnost at net.bio.net> From: Lim Pohyam <pohyam at technet.sg>
> Subject: precipitation problems
> Date: 4 Nov 1995 08:30:10 -0800
> Sender: daemon at net.bio.net> Message-ID: <199511041628.AAA05390 at einstein.technet.sg>
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>> My bacterial recombinant proteins precipitates upon long storage at -20C.
> Upon thawing from freezer, I detected strands of precipitates. There is
> difficulty to re-suspend these strands. As a result, when I set up my
> microtiter plates, the activity is decreased. I sometimes encounter as much
> as 15-20% drop in protein concentration. This is creating havoc in my
> assays. I would like to receive feedback on how to remove this problem. what
> can I do? I keep losing my protein everytime I thawed them from the freezer!
>>>>Have you tried freeze-drying your proteins and redissolving from these?
Dr Rob Harling
SAC (Scottish Agricultural College)/
University of Edinburgh
West Mains Road
Edinburgh EH9 3JG
Scotland, UK
tel: +44 (0)131 535 4000
fax: +44 (0)131 667 2601
e mail: esa009 at ed.sac.ac.uk
