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(Fwd) Re: commercial plant pathogen molecular diagnostics

James Miller millerj at knoxy.agvic.gov.au
Mon Jun 3 22:37:05 EST 1996

------- Forwarded Message Follows -------
Date:          Fri, 17 May 1996 00:46:59 -0700
From:          odonnell at sasa.gov.uk ("Kevin O'Donnell")
Subject:       Re: commercial plant pathogen molecular diagnostics
To:            diagnost at net.bio.net
Reply-to:      odonnell at sasa.gov.uk

On 16 May 96 at 22:35, James Miller wrote:

> I am interested in gathering information about commercial molecular 
> diagnostics for plant pathogens.
> As I understand there are many people researching and using molecular 
> diagnostics for plant pathogens around the world (specifically PCR 
> tests, dot blots, nucleic-acid based tests).
> However, not many of these tests have been successfully integrated 
> into commercial services.
> Is this indeed the case, and does anybody know of molecular tests for 
> plant pathogens that are currently being used in commercial 
> situations?

A bit of a potato centric answer for you I'm afraid:

I know of one company in the UK that uses dot blots to detect potato 
viruses in tubers.  We (Scottish Agricultural Science Agency) use dot blots
 to detect potato spindle tuber viroid in potato plants received in our 
quarantine facility.  

PCR is routinely used in the EC for testing for Pseudomonas 
solanacearum (Brown Rot of potatoes) and Clavibacter michiganensis 
subsp. sepedonicus (Ring Rot of potatoes). 

There are also PCR-based tests for potato cyst nematode 
identification that are coming into routine use.

If you get any replies by email, please repost the information here 
as this is a subject of interest to many of us.

Hope this is helpful.


Dr Kevin O'Donnell                          "I'm happy, I'm happy
Diagnostics and Molecular Biology    and I'll punch the man that says I'm not"
SASA                                                   - Ivor Cutler

James Miller
Agriculture Victoria
Institute for Horticultural Development
Private Bag 15
South Eastern Mail Centre
Victoria 3176

Ph-(03) 9210 9252             (o o)
FAX- (03) 9800 3521          ( O )

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