Dear Diagnostics Newgroup Members:
A question was recently posted regarding alternate sources for two-part TMB
liquid substrates for ELISAs using HRP conjugates. After enjoying the
convenience and reliability of one-part TMB substrates for a few years, I am
curious as to why anyone would stick to using two-part substrates. Have any
of you found any differences in sensitivity or linearity between the two
types of TMB? I know that some brands of one-part substrates may suffer from
a fairly short shelf life, but I have used two brands that last for over a
year under refrigeration, one of which also lasts at least six months without
refrigeration. The latter comes two forms, to produce either a soluble
product or a precipitate, both of which work equally well.
Mark Hughes
MHughes77 at aol.com
