Does any body have any thoughts on the useful shelf life of ELISA
reagents. I've recently been doing a series of experiments comparing the
sensitivity of various ELISA protocols. One thing I have noticed, which
is slightly annoying, is that on opening a new tin of skimmed milk
(Marvel) and a new bottle of tween-20 (BDH) there are big reductions in
the background. OK, so this may have been expected, but not to the extent
that I have noticed. Both of the 'old' reagents had probably been opened
less than 6 months ago. Any tips on storage etc?
Regards
Dave
