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clubroot confirmation

Rob Harling r.harling at ed.sac.ac.uk
Tue Jun 17 10:37:20 EST 1997

On 17 Jun 97 at 5:58, M. L . Putnam wrote:

> To:            nobody at net.bio.net
> Date:          Tue, 17 Jun 1997 05:58:47 -0700
> From:          "M. L . Putnam" <putnamm at ava.bcc.orst.edu>
> Subject:       clubroot confirmation

> I believe this may have come up once already but will risk repeating an inquiry:
> Does anyone have a serological assay for Plasmodiophora brassicae?  Or some
> other means of confirming infection in asymptomatic plants?  I can see the
> plasmodia in roots with no symptoms and would like some other means of
> confirming the diagnosis without having to wait for clubs to form.
> Any help would be appreciated.
> M.L. Putnam
> Extension Plant Pathology
> Oregon State University

If your plants originate from field soil, the plasmodia present in the 
roots may equally be those of Olpidium sp - this is a common soil fungus 
(or rather soil Protist - I guess the Plasmodiophoromycetes are no longer 
considered to be fungi).

Immunofluorescence detection methods do exist:
1.  Arie T et al 1988.  Annals of the Phytopathological Soc of Japan 54, 
242 - 245.

2.  Wakeham, AJ and White, JG 1996. Physiological and Molecular Plant 
Pathology 48, pages ? - ?  You might like to ask the author of this paper 
about obtaining antibodies:  Geoff White, at Horticulture Research 
International, Wellesbourne, Warwick CV35 9EF, UK.  

Good luck with this recalcitrant beast,
Rob Harling

Dr Rob Harling
SAC (Scottish Agricultural College)/
University of Edinburgh
West Mains Road
Edinburgh EH9 3JG
tel +44 (0)131 535 4000
fax +44 (0)131 667 2601
e mail r.harling at ed.sac.ac.uk

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