On 17 Jun 97 at 5:58, M. L . Putnam wrote:
> To: nobody at net.bio.net> Date: Tue, 17 Jun 1997 05:58:47 -0700
> From: "M. L . Putnam" <putnamm at ava.bcc.orst.edu>
> Subject: clubroot confirmation
> I believe this may have come up once already but will risk repeating an inquiry:
>> Does anyone have a serological assay for Plasmodiophora brassicae? Or some
> other means of confirming infection in asymptomatic plants? I can see the
> plasmodia in roots with no symptoms and would like some other means of
> confirming the diagnosis without having to wait for clubs to form.
>> Any help would be appreciated.
>> M.L. Putnam
> Extension Plant Pathology
> Oregon State University
If your plants originate from field soil, the plasmodia present in the
roots may equally be those of Olpidium sp - this is a common soil fungus
(or rather soil Protist - I guess the Plasmodiophoromycetes are no longer
considered to be fungi).
Immunofluorescence detection methods do exist:
1. Arie T et al 1988. Annals of the Phytopathological Soc of Japan 54,
242 - 245.
2. Wakeham, AJ and White, JG 1996. Physiological and Molecular Plant
Pathology 48, pages ? - ? You might like to ask the author of this paper
about obtaining antibodies: Geoff White, at Horticulture Research
International, Wellesbourne, Warwick CV35 9EF, UK.
Good luck with this recalcitrant beast,
Dr Rob Harling
SAC (Scottish Agricultural College)/
University of Edinburgh
West Mains Road
Edinburgh EH9 3JG
tel +44 (0)131 535 4000
fax +44 (0)131 667 2601
e mail r.harling at ed.sac.ac.uk