snip . . .from Howie Rose:
>Has anybody had experience of using RNA probes for the detection of
pathogenic bacteria without an amplification system?
------------
Isn't that the basis of the initial (and currently available) GenProbe
manufactured commercial "DNA" probe tests - going for RNA of the subject
pathogens rather than DNA? Their combination GC/Chlamydia test has been
well accepted in clinical lab settings, along with other probe-based assays
that they make using a similar "RNA" test principle, I believe. No
amplification is used other than the "natural" application for those
assays. (They do have an amplifications system, but amplification is not
utilized for the subject assays.)
Cole Owen
Owen & Associates, Inc.
Consultants to the Healthcare Industry
1233 Camino Del Mar
Del Mar, California, USA
phone (vox) 619-481-5991
phone (fax) 619-481-1741
email: cowen at electriciti.com
