We had pretty good luck just boiling spore suspensions in TE. We got
good sensitivity down to somewhere around 4-5 spores/PCR for
Magneporthe grisea.
On 15 Sep 98 at 12:09, Kevin O'Donnell wrote:
> Can anyone suggest a good (i.e. rapid, simple) method for isolating
> DNA from fungal spores? The quality of DNA has only to be good enough for
> PCR or LCR ampliciation, rather than cloning, orsequencing.
>> Replies to the list please, rather than private email.
>> Thanks,
>> Kevin
> Dr Kevin O'Donnell
> Diagnostics and Molecular Biology
> Scottish Agricultural Science Agency Tel 0131 244 8924
> Edinburgh Fax 0131 244 8926
> EH12 8NJ UK
>>
PLEASE NOTE THAT MY EMAIL HAS CHANGED TO DRHOADS at COMP.UARK.EDU
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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"Strong and Bitter Words Indicate a Weak Cause."
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Dept. of Biological Sciences, University of Arkansas
drhoads at comp.uark.edu, http://comp.uark.edu/~drhoads
501-575-3251
