Dear Dr. O'Donnell,
You might find these two references to be helpful;
1) Lee, S.B. and Taylor, J.W. 1990. Isolation of DNA from fungal
mycelia and single spores. PCR protocols: A Guide to Methods and
2) Kuske c.R. et al.,1998. Small-scale DNA sample preparation Method for
field PCR detection of microbial cells and spores in soil. Appl Environ
Microbiol vol 64 (7): 2463-2472.
Hope this helps.
Yatika Kohli, Ph.D.
Ontario Ministry of Health
Etobicoke, ON Canada.
>From biosci-request at net.bio.net Tue Sep 15 04:58:33 1998
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>To: nobody at net.bio.net>X-Really-To: diagnost at net.bio.net>Newsgroups: bionet.diagnostics
>From: "Kevin O'Donnell" <odonnell at sasa.gov.uk>
>Organization: Scottish Agricultural Science Agency
>Date: Tue, 15 Sep 1998 12:09:42 +0000
>Subject: DNA isolation from fungal spores
>>Can anyone suggest a good (i.e. rapid, simple) method for isolating
>DNA from fungal spores? The quality of DNA has only to be good enough
>PCR or LCR ampliciation, rather than cloning, orsequencing.
>>Replies to the list please, rather than private email.
>Dr Kevin O'Donnell
>Diagnostics and Molecular Biology
>Scottish Agricultural Science Agency Tel 0131 244 8924
>Edinburgh Fax 0131 244
>EH12 8NJ UK
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