Dear colleagues
We use PCR-RFLP of mtDNA to successfully distinguish several thrips (=
"tiny insects") species. Our protocols work well with fresh, frozen or dry
specimens. However, some have been caught and stored on commercially
available glue traps (,tanglefoot" ?), and we have a hard time to get a
reasonable PCR product from these DNA extractions. We suspect that the glue
on the traps inhibits the PCR reaction.
We tried to remove the glue mechanically and with benzine, with little
success. Diluting the DNA is problematic too, because exctractions often
yield not more then pico-grams / ul. Has anyone similar experiences and a
solution for this problem? Any input is highly appreciated!
Sincerely,
Patrick Brunner
---------------------Dr. Patrick C. Brunner------------------
Swiss Federal Research Station
Schloss, Lab. 4 phone: +41 (1) 783 6331
CH-8820 Waedenswil fax: +41 (1) 783 6434
Switzerland e-mail: patrick.brunner at faw.admin.ch
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