Hi Tim,
we have been using the Boehringer Mycoplasma Detection kit
for a number of years to ensure that our hybridoma stocks are mycoplasma
free. The test is based on a DAS avidin/biotin ELISA
of tissue culture supernatant and is quick and easy to use.
We messed about with the Life Technologies Mycotect kit which uses
an indicator cell line (3T3) which is sensitive to methylpurine
and methylpurine riboside. This product is converted from non-toxic
methyl purine deoxy riboside by the action of adenosine phosphorolase
(produced by mycoplasma). The test takes 5-6 days against 1 day for the
Boehringer system. It's main advantage is that it will detect all
mycoplasma
instead of the "big four" that
the Boehringer system identifies.
We also tried the Hoechst mycoplasma stain system but found it very
difficult to interpret as you are basically looking for tiny amounts of
extra-nuclear mycoplasma DNA (which stain positive with the dye).
Hope this is of use to you
Regards
Robert
-----Original Message-----
From: tritty [mailto:tritty at cellbio.wustl.edu]
Sent: 06 October 1999 21:21
To: bionet-diagnostics at net.bio.net
Subject: micoplasma kits?
can anyone recommend a kit for the detection of micoplasma in tissue
culture
reagents?
thanks in advance,
Tim
------------------------------------------------------------------------
----
----------------------
Timothy M. Ritty, Ph.D.
(314) 362-2211
Washington University
Dept. of Cell Biology, Box 8228
660 South Euclid
St. Louis, MO
63110
