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detection of viable moulds on RNA basis

daemon at hgmp.mrc.ac.uk daemon at hgmp.mrc.ac.uk
Wed Feb 9 07:49:48 EST 2000

Does anybody know the half life time of free ribosomal DNA in a 
neutral solution?
My idea is to detect in a quantitive way the amount of viable 
moulds on basis of RNA. The idea is that RNA has a short half life 
and therefor a RT PCR of freshly isolated total RNA could tell 
something about the number of viable cells. However I am not sure 
about the stability of ribosomal RNA.


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