Hi
we regularly assay for the t(14;18) rtanslocation which involves the
bcl2 oncogene and the IgH gene. Our standard positive control is a PCR
product of approximately 230bp. recently we obtained a patient sample
which gave a product of over 1000 bp.
My question is: how big do they get? is it possible that a false
negative could be obtained for very large and possibly non amplifiable
products. Is there a diagnostic significance for very large rearranged
products?
thanks for your help
Louise
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