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GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP |
i make amplification with primers that give a 700bp fragment. i had tried to make migration in 25 to 35% dénaturant gel at 75°C but for three time i had obtained no séparation and the last time there was no band. can you explain me this or can you show me a reference for this thank you ---