There are major and minor breakpoints at this locus.
The rearrangement involves slippage along some Alu
(repetitive) DNA in the heavy chain gene. You should
read the literature. There are also indications that
normal individuals contain a small percentage of cells
which rearrange. PNAS paper around 1994-95 if I remember
correctly. So you need to really, really know what you
are looking for at this locus before coming to any
conclusions.
Good luck.
Bert Gold, Ph.D.
Sherman Oaks, California
daemon at hgmp.mrc.ac.uk, [mailto:daemon at hgmp.mrc.ac.uk], On, Behalf, Of
wrote:
>> Hi
>> we regularly assay for the t(14;18) rtanslocation which involves the
> bcl2 oncogene and the IgH gene. Our standard positive control is a
PCR
<lots of stuff deleted>
