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identification of rDNA

nobody at hgmp.mrc.ac.uk nobody at hgmp.mrc.ac.uk
Thu Nov 16 07:40:26 EST 2000

Dear colleagues,
I want to check plasmid inserts in a Southern HYB for clones with 
repetitive sequences. So I am planning to hybridyze with maize 
genomic DNA to identify these rDNA containing clones. I am not 
sure about the amount of labelled probe to use for this HYB. For a 
normal Southern with a certain fragment as probe i use 50-100ng 
(P32-labelling). But is this enough to cover all species of rDNA 
within genomic DNA?
Thanks for your answers in advance,



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