March 2004 Newsletter

[qPCR.Gene-Quantification.Info Newsletter]

Gene Quantification Newsletter March 2004

New URLs were defined for the Gene Quantification Main page

<http://www.gene-quantification.info/>http://www.gene-quantification.info
or
<http://qpcr.gene-quantification.info/>http://qpcr.gene-quantification.info
or
<http://www.real-time-pcr.info/>http://www.real-time-pcr.info



BioInformatics in real-time PCR

and
Determination of real-time PCR efficiency
<http://bioinformatics.gene-quantification.info/>http://bioinformatics.gene-quantification.info




Endocrinology, Physiology and Immunology

<http://physiology.gene-quantification.info/>http://physiology.gene-quantification.info




The 1st qPCR Symposium & Application Workshop talks are FREE for download

<http://qpcr2004.gene-quantification.info/>http://qpcr2004.gene-quantification.info
and
<http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html>http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html


New features on the Gene-Quantification web page:

Determination of real-time PCR efficiency
<http://www.gene-quantification.info/efficiency.html>http://www.gene-quantification.info/efficiency.html

PCR Efficiency estimation via "experimental four parametric sigmoidal model 
fit" (including all fluorescence data points, Tichopad et al. MCP 2004 - 
Inhibition of real-time RTPCR quantification due to tissue-specific 
contaminants)
<http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-4>http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-4

PCR Efficiency estimation via "experimental four parametric logistic model 
fit" (including all fluorescence data points, Tichopad et al. 2003 - 
Standardized determination of real-time PCR efficiency from a single 
reaction set-up.)
<http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-43>http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-3

KOD (kinetic outlier detection) in real-time PCR (Tzachi Bar et al., 2003)
<http://www.gene-quantification.info/efficiency.html#kod>http://www.gene-quantification.info/efficiency.html#kod

Effect of zinc deficiency on the mRNA expression pattern in liver and 
jejunum of adult rats: Monitoring gene expression using cDNA microarrays 
combined with real-time RT-PCR
<http://www.gene-quantification.info/zinc.html#array1>http://www.gene-quantification.info/zinc.html#array1

Identification of Genes Responsive to Intracellular Zinc Depletion in the 
Human Colon Adenocarcinoma Cell Line HT-29
<http://www.gene-quantification.info/zinc.html#array2>http://www.gene-quantification.info/zinc.html#array2

The mRNA Expression of TWO Insulin Receptor Isoforms (IR-A and IR-B) and 
IGFR-2 in the Bovine Corpus Luteum During the Estrous Cycle, Pregnancy, and 
Induced Luteolysis
<http://www.gene-quantification.info/endo.html#insulinrec>http://www.gene-quantification.info/endo.html#insulinrec

Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in real-time PCR, new quantification 
strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE 
to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact 
<mailto:gene.quantification at wzw.tum.de>gene.quantification at wzw.tum.de   You 
will receive all NEW FEATURES and future REST &  REST-XL updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are 
non-commercial, educational sites with the only purpose of facilitating 
access to biology-related information over the internet. The web page is 
optimised for Netscape Navigator version 4.7 & Internet Explorer version 
5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 
pixels.

********************************************************************************************
Gene-Quantification.Info

mail:           gene.quantification at wzw.tum.de
homepage:       http://www.gene-quantification.info

PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY
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