Gene Quantification Newsletter May 2004
A new URL is defined for the Gene Quantification home page
<http://www.gene-quantification.info/>http://www.gene-quantification.info
A lot of NEWS are presented:
On HKG and normalization page:
Guideline to reference gene selection for quantitative real-time PCR
Selection of appropriate control genes to assess expression of tumor
antigens using real-time RT-PCR
Comparison of RiboGreen® and 18S rRNA quantitation for normalizing
real-time RT-PCR expression analysis
Novel Internal Controls For Real-Time PCR Assays
Selection of optimal internal controls for gene expression profiling of
liver disease
On real-time PCR page:
Real-time PCR in the microbiology laboratory
Quantitative RT-PCR: pitfalls and potential
Real-time RT-PCR: Neue Ansätze zur exakten mRNA Quantifizierung
On chemistry page:
<http://dyes.gene-quantification.info/>http://dyes.gene-quantification.info/
Short summary of detection formats
SYBR Green I
BEBO
TaqMan Probes
LightCycler Hybridisation Probes
Molecular Beacons
Scorpions
LNA Primers
Light-Up Probes
Q Zyme Assay
Amplifluor Quantitative PCR Detection System
LUX (Light Upon eXtension) primers
The iCycler iQ Detection System for multiplex real-time PCR Assays
A novel real-time quantitative PCR method using attached universal template
probe
Determination of real-time PCR efficiency
All necessary informations about qPCR efficiency estimation
<http://efficiency.gene-quantification.info/>http://efficiency.gene-quantification.info
BioInformatics in real-time PCR
A lot of applications related to qPCR or real-time PCR
STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED
<http://bioinformatics.gene-quantification.info/>http://bioinformatics.gene-quantification.info
Endocrinology, Physiology and Immunology
Application of real-time PCR in molecular Physiology, molecular
Endocrinology and molecular Immunology
<http://physiology.gene-quantification.info/>http://physiology.gene-quantification.info
The 1st international qPCR Symposium & Application Workshop
Please look at the nice pictures of the Symposium
<http://qpcr2004.gene-quantification.info/>http://qpcr2004.gene-quantification.info
and Workshop talks are FREE for download
<http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html>http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html
Please send the NEWSLETTER and the
<http://www.gene-quantification.info/>http://www.gene-quantification.info
home page links to further scientists and friends who are interested in
real-time PCR, new quantification strategies, relative or absolute gene
quantification using kinetic PCR.
best regards
Michael Pfaffl
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Disclaimer & copyright on
<http://www.gene-quantification.info/>http://www.gene-quantification.info
homepage.
********************************************************************
www.Gene-Quantification.Info
mail: gene.quantification at wzw.tum.de
homepage: http://www.gene-quantification.info
PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan, GERMANY
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