I am carrying out an experiment about water stress in sclerophyllus
plants (quercus coccifera and pistacia lentiscus). I would like study
limitations at CO2 fixation level related with cell dehydration. For
this purpose, I will measure photosynthesis with IRGA at normal CO2
concentration and at 2000 ppm concentration. The reason for high
concentration is because I think that at this concentration, CO2
diffuses through cuticle and other leaf barriers when stomata are
closed. And, I will able differentiate between limitations to
photosynthesis due to stomatal closure or damage at photosynthetic
machinery level because of cell dehydration. My question is if 2000
ppm will be sufficient CO2 concentration to diffuses through cuticle
when stomata are closed or I will need to use higher concentration?.
Any comment will be gratefully received. Thanks in advanced.
A.Vilagrosa
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Alberto VILAGROSA
Dept. Ecologia-CEAM. Univ. Alicante
POBox 99. 03080 Alicante. SPAIN
Tel. 34-96 59 03 400
Fax. 34-96 59 03 464
e-mail: albertov at ctv.es