shender at macc.wisc.edu (Sam Henderson) wrote:
>> Does any one know how long it takes GFP to fold? Various people
> have told me anything from 1-4 hrs. I'm using the mutated form
> of GFP found in Andrew Fires series of C. elegans vectors. It seems
> that this would be a concern if I am looking at timing of expression.
> And does anyone have a reference on this. Thanks in advance.
Which GFP mutation is in Andrew Fire's vectors? The S65T
chromophore mutation described by Heim et al (1995) in
Nature 373:663-4, is reported to have a half-time for chromophore
formation as approx. 30 mins (see fig 2b), compared to 2 h for wt.
-= Paul =-
! Paul Kitts !
! CLONTECH Laboratories, Inc. !
! 1020 East Meadow Circle Email: pakitts at clontech.com !
! Palo Alto Phone: 415 424 8222 x1463 !
! CA 94303-4230, U.S.A. Fax: 415 424 1064 !