Dear GFP users,
I am not the first trying double GFP/DAPI "staining" of
C. elegans worms. I have heard that methanol/acetone
and formaldehyde fixation work. Unfortunately I haven't
been able to keep the GFP staining with the methanol/ace-
tone fixation of young larvae.
I would be grateful too to know if some of you have been
able to find photography conditions better than mine:
Fudji PROVIA reversal films (400 to 1600 ASA), 3' of exposition!!
Thank you in advance for any advice,
email: claudia.wittmann at unifr.ch