Does anyone have experience using the molecular probes Imagene green
and/or Imagene red for plant tissue (not cell culture)? We are interested
in information concerning the overlap of the fluorescence with endogenous
molecules like chlorophyl and on the permeability of these GUS substrates
into tissue. We are specificaly interested in using these substrates to
detect GUS activity in pollen tubes in the style or in-vitro germinated
but any information will be helpful.
thanks in advance. Yoram Eyal and Jorge Muschietti, McCormick lab - UCB.