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GFP Western

ROSBF ravenn at ix.netcom.com
Wed Oct 25 10:06:14 EST 1995


jjschm at ccmail.monsanto.com (Jon Schmuke) gave us this to ponder:

>Has anyone tried or considered using gfp as a western reagent?
>I was curious to know if a gfp-biotin system would provide high level
>non-radioactive sensitivity.  I'm not sure how this would work with the UV
>requirement for activation, but you can certainly take a picture of the
>fluoresence so there should be a way to make this work.

>Food for thought...

>Comments?

Interesting.

Of course, you can use the antibody from Clonetech and do the Western
the "old fashioned way" 8).

The ab works for this BTW.

R.





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