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Variable visually detectable fluorescence from GFP

Michael Moser moser at U.WASHINGTON.EDU
Wed Jan 3 01:26:50 EST 1996


This response seems similar to the experience that I have 

Mike Moser                                             Tel: 206-543-5354
UW Department of Biochemistry                          FAX: 206-685-1792
Box 357350                                        moser at u.washington.edu
Seattle, WA  98195-7350                     Make peace my beast is yeast

On Tue, 2 Jan 1996, Brandt Cassidy wrote:

> In article <4c1fv5$jrm at itssrv1.ucsf.edu>, Brian Wickes
> <wickes at cgl.ucsf.edu> wrote:
> 
> > Has anyone had difficulty expressing GFP-1 in bluscript?  How did you 
> > make your construct (using SK+ and GFP from pGFP-1) and was a longwave 
> > hand held UV light ok?
> > 
> > Brian
> 
> We found that only some of the colonies from the transformation gave very
> high fluorescence. We did use a hand held UV light source to detect the
> Fluorescence.  It helps to have a dark room to see it. A nontransformed E.
> coli control is good for comparison as well.
> 
> Brandt G. Cassidy
> Assistant Scientist
> Plant Biology
> The Samuel Roberts Noble Foundation
> e-mail  BGCASSIDY@ noble.org
> 
> 




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