wklimke at gpu.srv.ualberta.ca (William Klimke) wrote:
>Does GFP require an extraneous energy source to function, and if so, can this
>energy source (I'm assuming that it's ATP) be supplied for example in the
>media, or does it need to be supplied by the cell. I'm considering doing a
>fusion to an outer membrane protein, so it would not have a readily available
>source of energy.
> It is my understanding that GFP requires only molecular oxygen and time to fold into the proper configuration to work.