The "leakage" of GFP after fixation with ethanol poses a problem with
concomitant analysis of GFP+ cells and DNA content with PI. But this
does not appear to be a problem with Hoechst 33258, which is used to
stain live cells and doesn't require the cell membrane to be permeabilized.
Has anyone tried this approach or see any reason this won't work?
This will obviously need two lasers.
Please post, or email and I will summarize the responses.