it is quite possible that the tube absorbs too much uv exciting light.
try to avoid it. and try again.
hope you see the glowing green.
hospital for sick children
Mar 1996, Rainer H Kohler wrote:
> I grow GFP in TB at 37 C and don't see fluorescence of cells in the culture
> tube. But the cell pellet shows nice fluorescence visible when exposed to
> a 365 nm hand held UV source.
> Dr. Rainer H. Koehler
> Department of Genetics & Development
> 321 Biotechnology Building home
> Cornell University 80 Lois Lane
> Ithaca, NY 14853, USA Ithaca, NY 14850
> phone lab: 607 254 4832 phone home: 607 273 4068
> FAX: 607 255 6249
> e-mail:rhk2 at postoffice.mail.cornell.edu> __________________________________________________________________