Hi, A while ago someone wrote into this news group describing the minitoring
of GFP fluorescence using a fluorimeter in a process which required washing
the cells expessing the GFP with buffer prior to reading the fluorescence.
this avoided problems of fluorescence of LB. Unfortunately our computer
system crashed and I lost the details of the buffer which the person had
kindly sent me. Thus I would be very grateful to receive the details again,
and also the wavelenghts used for optimal excitation. (We were exciting at
395nm and monitoring the emission at 509nm).
Thanking you in advance,