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Non-green fluorescent protein

Gene Regulation "grggta at picr.cr.man.ac.uk, grglp" at picr.cr.man.ac.uk
Wed Nov 27 12:24:01 EST 1996


I would like to use a fluorescent protein as a marker for DNA uptake in
transient assay (mammalian cells) but need to counterstain the cells for
a second protein with FITC ie green! Is there a red or blue fluorescent
protein which is easy to see using a fluorescent microscope? 

Secondly, I have tried using S65T with little success in COS and 3T3
fibroblasts. Fluorescence is there but is weak - we transfect with
sv40-driven S65T and 24 - 48 hours later look at the cells (growing on
slides) under FITC illumination - the cells are still in DMEM and are
viable. Can anyone give me a good protocol for using GFP?

Many thanks
G Atherton
reply to grggta at picr.cr.man.ac.uk



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