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GFP and immunofluorescence

Stephan Geley s.geley at ICRF.ICNET.UK
Fri Apr 4 18:33:35 EST 1997

I'm looking for some advive how to fix cells for immunostaining without
loosing a GFP signal. So far, I' ve tried either methanol,
methanol/acetone, or paraformaldehyde/TX100. However, none of these
methods worked and I always loose the very bright GFP signal that I
obtain in living cells.
Thanks for your help,

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