IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

GFP and immunofluorescence

Stephan Geley s.geley at ICRF.ICNET.UK
Fri Apr 4 18:33:35 EST 1997


I'm looking for some advive how to fix cells for immunostaining without
loosing a GFP signal. So far, I' ve tried either methanol,
methanol/acetone, or paraformaldehyde/TX100. However, none of these
methods worked and I always loose the very bright GFP signal that I
obtain in living cells.
Thanks for your help,
Stephan



More information about the Fluorpro mailing list

Send comments to us at archive@iubioarchive.bio.net