Your best options are:
EGFP from Clontech, max fluorescence at about 405 nm, still bright at 490
mGFP/GFP5 from Haseloff. See: K.R. Siemering, R. Golbik, R. Sever, J.
Haseloff (1996) Mutations that suppress the thermostability of green
fluorescent protein. Current Biology 6(12):1653-1663. (see article for
>>We are intending to create a recombinant plant virus containing a reporter
>gene in order to locate the progress of infection. Colleagues of us will
>soon buy a fluorescence microscope that we'll be allowed to use, however
>we'll more probably routinely use a hand-held UV lamp.
>>Could anyone please help us in the choice of the optimal GFP version to use
>(wt, EGFP, S35T, uv, other...).
>>Thanks for your help.
>Olivier Le Gall
> Pathologie Vegetale, INRA Bordeaux, BP 81 :
> 33883 Villenave d'Ornon Cedex, FRANCE -,o
>Tel +33(0)556 843 205 Fax +33(0)556 843 222 ( )
>legall at bordeaux.inra.fr /----"-"---
>* * * * * * *-* *-*-*-*-*-*-*-*-*-*-*-*-----/ W
George McNamara, Ph.D.
Universal Imaging Corporation
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