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GFP fusion protein in CHO

R. Maney toddman at U.WASHINGTON.EDU
Wed Apr 30 12:11:22 EST 1997


with our experience, GFP alone in CHO cells does not aggregate in any
specific structure but localizes to both the cytoplasm and nucleus
uniformly. One note: our pictures seem to show slightly darker staining
in the nucleus than the cytoplasm but this could be due to cell geometry
rather than a property of GFP. GFP, given its small size, is expected to
enter the nucleus via free diffusion.

todd


On 30 Apr 1997, Beatrice Marqueze wrote:

> I have constructed a fusion protein with GPF using Clontech vector
> pEGFP-N1. My goal is to study in CHO cells the distribution of the fusion
> protein in the different sub-cellular compartments.
> 
> Does anybody know where GFP alone is distibuted when transfected in CHO, in
> the cytoplasm, in the nucleus ? Could the  fusion protein be wrongly
> localized in the GFP compartments?
> 
> 
> ..............................................................................
> Beatrice Marqueze-Pouey
> INSERM U 374/464, Institut Federatif Jean Roche, Faculte de Medecine-Nord,
> 13916 Marseille Cedex 20, France.
> Tel (33) 4 91 69 88 32, Fax (33) 4 91 09 05 06,
> E-mail: marqueze at jean-roche.univ-mrs.fr
> ............................................................................
> ..
> 
> 
> 
> 




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