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EGFP-Permeablizing cells

MHoust1000 at aol.com MHoust1000 at aol.com
Wed Aug 6 11:20:54 EST 1997


Hi,
Can anyone tell me if permeablizing cells that have been transfected with GFP
(Clontech's EGFP) for the purpose of staining them with other fluorochromes
will have any adverse effects on GFP?  I want to use 0.1% Triton on the cells
and let them sit overnight with it.

Thank you,

Michele Houston
Albert Einstein Cancer Center 
Montefiore Medical Center
Department of Oncology



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