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EGFP-Permeablizing cells

Julie R. Hens jrhens at WAM.UMD.EDU
Thu Aug 7 07:19:56 EST 1997


Steffen, when you say " before there was a fixation with formaldehyde",
does this mean, that you viewed the GFP before fixing it? but after the
triton X treatment?
thanks for the response,
Julie

On 7 Aug 1997, Steffen Dietzel wrote:

> I used higher concentrations of Triton on Drosophila tissues over the
> week-end once. The GFP was fine afterwards. However, before there was a
> fixation with formaldedehyde. 
> 
> Steffen Dietzel
> 
> 
> In article <970806121933_-936662085 at emout19.mail.aol.com>,
> MHoust1000 at aol.com wrote:
> 
> > Hi,
> > Can anyone tell me if permeablizing cells that have been transfected with GFP
> > (Clontech's EGFP) for the purpose of staining them with other fluorochromes
> > will have any adverse effects on GFP?  I want to use 0.1% Triton on the cells
> > and let them sit overnight with it.
> > 
> > Thank you,
> > 
> > Michele Houston
> > Albert Einstein Cancer Center 
> > Montefiore Medical Center
> > Department of Oncology
> 
> -- 
> Steffen Dietzel
> Zentrum fuer molekulare Biologie Heidelberg (ZMBH)
> University of Heidelberg
> Im Neuenheimer Feld 282
> 69120 Heidelberg, Germany
> Phone: +49/6221/54-6836 or -6879. Fax: -5893
> listservmail:dietzel at sun0.urz.uni-heidelberg.de
> personal mail: dietzel at wotan.iwr.uni-heidelberg.de
> 
> 




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