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EGFP-Permeablizing cells

Steffen Dietzel dietzel at sun0.urz.uni-heidelberg.de
Thu Aug 7 06:43:30 EST 1997


I used higher concentrations of Triton on Drosophila tissues over the
week-end once. The GFP was fine afterwards. However, before there was a
fixation with formaldedehyde. 

Steffen Dietzel


In article <970806121933_-936662085 at emout19.mail.aol.com>,
MHoust1000 at aol.com wrote:

> Hi,
> Can anyone tell me if permeablizing cells that have been transfected with GFP
> (Clontech's EGFP) for the purpose of staining them with other fluorochromes
> will have any adverse effects on GFP?  I want to use 0.1% Triton on the cells
> and let them sit overnight with it.
> 
> Thank you,
> 
> Michele Houston
> Albert Einstein Cancer Center 
> Montefiore Medical Center
> Department of Oncology

-- 
Steffen Dietzel
Zentrum fuer molekulare Biologie Heidelberg (ZMBH)
University of Heidelberg
Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Phone: +49/6221/54-6836 or -6879. Fax: -5893
listservmail:dietzel at sun0.urz.uni-heidelberg.de
personal mail: dietzel at wotan.iwr.uni-heidelberg.de



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