pKEN contains GFPmut1, 2 & 3 made by Cormack (Cormack, B.P., Valdivia, R.
H. & Falkow, S. Gene 173:33-38) which are some of the brigthest GFP around.
Information about pKEN can be found in Ezaz-Nikpay, K., Uchino, K., Lerner,
R.E. & Verdine, G.L. Protein Science 3:132-138.
You can probably get the plasmids from Dr Cormack. If you have any more
questions just send me an email.
>>My name is Scott, and I am a reader of the fluro-pro newsgroup. Much
>earlier in the year, I remember someone mentioned that they were using the
>pKEN plasmids with their GFP constructs. I was wondering what are the
>attractions and details of these plasmids, plasmid maps etc, as I have not
>been able to find out any information about them, or where they can be
>purchased from. Any information would be most helpful.
>>>>>At 09:01 AM 2/26/97 -0000, you wrote:
>>>>Do you know if there is any difference between pKEN and pKEN2? I use the =
>>GFP-plasmids designed by Cormack and he's using pKEN
>>ngn at lysator.liu.se>>Phone +046 (0)13-14 19 47 (home)
>> +046 (0)13-22 20 56 (University)
>>Division Of Animal Health
>Australian Animal Health Laboratory
>>Private Bag 24 (5 Portarlington Road)
>Geelong Victoria 3220 Australia
>Ph: 03 5227 5761
>Fax: 03 5227 5531
>email: scott.sheedy at aahl.dah.csiro.au>http://www.csiro.au