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GFP visualization in Arabidopsis

Natasha Myers NMYERS at BIO1.Lan.McGill.CA
Thu Aug 7 15:22:25 EST 1997


Hello,
I am hoping to use GFP as a marker for gene activity in specific cell 
layers of chimeric plants.  We have no problem seeing mGFP5 in the 
root (and petals), but cannot resolve GFP fluorescence from background 
autofluorescence in the leaves, sepals and other chlorophyll 
containing organs. Is there a special filter to use on a standard 
microscope, or apparatus (other than confocal) which would allow 
quick characterization?

Thanks in advance for your suggestions,

Natasha Myers

Sieburth Lab
McGill University
Montreal, PQ



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