IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

viewing GFP protein on Western blot

Alex Chang lpss at unixg.ubc.ca
Tue Aug 12 16:34:53 EST 1997


I am planning to do immunoprecipitation of my GFP-tagged protein, after 
SDS-PAGE and transblotting onto a membrane, look at the GFP-tagged protein 
directly by a UV light source without using antibody detection (or simply 
look at the gel directly without transblotting).

I'd like to know anyone has done this, and what kind of UV source can be 
used.


Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca



More information about the Fluorpro mailing list

Send comments to us at archive@iubioarchive.bio.net