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egfp reporter and redox

agnes agnesg at physiol.unizh.ch
Wed Aug 13 08:47:28 EST 1997


Hi,
I am trying to use EGFP as a reporter linked to a redox sensitive
promoter. As I never used EGFP before I have several questions:
First, does anyone has experience with electroporation? Or would
lipofection would be preferable? 
Second, does anyone know if mild reducing environments would affect egfp
expression or fluorescence? What about anoxia?
I greatly appreciate any help and comment.
Thanks a lot.
Agnes

Agnes Goerlach
University of Zurich 
Institute for Physiology
Winterthurerstr. 190
CH-8057 Zurich
Tel. +41 1 6355062
Fax. +41 1 6356814
e-mail: agnesg at physiol.unizh.ch



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