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Detaction of BFP signal

Incheol Shin icshin at BIONEER.KAIST.AC.KR
Mon Aug 18 20:47:37 EST 1997


I  am working with BFP or GFP fusion proteins. Using this proteins I
want to study calcium signal in cell. How I descriminate GFP signal from
fluo-3 (calcium signal)?  How can I detact BFP signal using MRC 1024?
Please give me information about filter sets.

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> FLUORESCENT-PROTEINS/bionet.molbio.proteins.fluorescent Newsgroup
> Archive
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> Please visit our sponsors' WWW pages and tell them you saw their ad on
> BIOSCI.
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> [GFP from Clontech, Inc.]  [Quantum Biotechnologies Inc.]
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>  The Best & Brightest GFP Products    Turn bright ideas to bright
> results
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> BIOSCI Home Page | Other Newsgroups | Journals Archive | BIOSCI
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> FLUORESCENT-PROTEINS/bionet.molbio.proteins.fluorescent via the BIOSCI
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> Read the most recent
> FLUORESCENT-PROTEINS/bionet.molbio.proteins.fluorescent articles (Aug
> 97)
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> This archive is broken down by month (MM) and year (YY)
> The articles are stored in directories further below named YYMM.
> ----------------------------------------------------------------
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>
> newsgroup, enter your query below. freeWAIS supports the Boolean
> operators "and" and "not" and the truncation operator *. If multiple
> search terms are
>
> PCR and sequenc* not DNA
>
> will find messages that contain the text "PCR" and any words starting
> with "sequenc" (sequence, sequencing, etc.) but exclude messages
> containing the text "DNA
>
> Search for:
>
> Maximum hits returned:
>
>
>
> -
> -------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> [     ] Name                   Last modified     Size  Description
> ------------------------------------------------------------------
> --------------------------------------------------------------------------------------------
>
>  [DIR]   Parent Directory       18-Aug-97 18:25      -
>
>  [DIR]   9505/                  13-Nov-95 05:18      -
>
>  [DIR]   9506/                  13-Nov-95 05:18      -
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>  [DIR]   9507/                  13-Nov-95 05:18      -
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>  [DIR]   9508/                  13-Nov-95 05:18      -
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>  [DIR]   9509/                  13-Nov-95 05:18      -
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>  [DIR]   9510/                  13-Nov-95 05:18      -
>
>  [DIR]   9511/                  27-Dec-95 20:06      -
>
>  [DIR]   9512/                  29-Dec-95 12:27      -
>
>  [DIR]   9601/                  31-Jan-96 20:25      -
>
>  [DIR]   9602/                  29-Feb-96 07:32      -
>
>  [DIR]   9603/                  31-Mar-96 14:45      -
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>  [DIR]   9604/                  30-Apr-96 18:26      -
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>  [DIR]   9605/                  31-May-96 16:05      -
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>  [DIR]   9606/                  28-Jun-96 16:50      -
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>  [DIR]   9607/                  31-Jul-96 14:54      -
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>  [DIR]   9608/                  29-Aug-96 08:49      -
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>  [DIR]   9609/                  30-Sep-96 19:25      -
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>  [DIR]   9610/                  31-Oct-96 17:10      -
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>  [DIR]   9611/                  28-Nov-96 03:18      -
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>  [DIR]   9612/                  31-Dec-96 19:21      -
>
>  [DIR]   9701/                  30-Jan-97 13:37      -
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>  [DIR]   9702/                  28-Feb-97 12:21      -
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>  [DIR]   9703/                  31-Mar-97 06:08      -
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>  [DIR]   9704/                  30-Apr-97 21:31      -
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>  [DIR]   9705/                  31-May-97 04:45      -
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>  [DIR]   9706/                  30-Jun-97 16:34      -
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>  [DIR]   9707/                  29-Jul-97 14:36      -
>
>  [DIR]   9708/                  18-Aug-97 08:47      -






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