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EGFP expression in e. coli

Doug Pittman dpittman at ARETHA.JAX.ORG
Wed Aug 20 12:16:23 EST 1997


Has anyone expressed EGFP in e. coli? If so, how well does it 
fluoresce and what promoter was used?

Also, does anyone have any EGFP mutants available that fail to 
fluoresce? I would prefer to have one as close to the C-terminus as 
possible. I've made one construct that removes the last 4 AA from EGFP 
and I still see weak and unstable fluorescence in ES cells. For my 
experiments, I prefer to have no detectable expression.

Thanks,
Doug Pittman
dpittman at jax.org



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