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GFP immunization

Ugo Moens Ugom at fagmed.uit.no
Tue Jun 24 04:44:36 EST 1997


Has anybody tried to make his/her own antibodies against GFP or does
anyone have experience with plasmid inocculation with GFP expression
plasmids? Give such plasmid inocculation strong antigen-responses?

We have inocculated Balb/c mice with pGFP-C1 (one single injection with 50
micrograms in each leg) plasmid DNA. This resulted in weak anti-GFP
antibody response.

I appreciate your response.

Ugo Moens

Ugo Moens
University of Tromsø
Institute of Medical Biology
Dept. of Gene Biology
N-9037 Tromsø
email: ugom at fagmed.uit.no

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