This may be a basic question but have done a quick search and cannot
seem to find any information regarding the pH-dependence of GFP
fluorescence. I have expressed a fusion protein containing EGFP in Hela
cells and would like to try to measure fluorescence in a cell lysate.
Does anyone know whether pH is an important parameter in quenching GFP
Any information greatly appreciated.
Dept. Cell Biology and Physiology
University of Pittsburgh
whill+ at pitt.edu