I am trying to use GFP as a control for my transformation efficiency. I am
using transformed human B cells. My problem is that I am having trouble
quantitating my flourescence. By microscopy I can detect some flourescence
but when I try to do FACS analysis I can't detect anything. I am using
EGFP and for the FACS analysis I am using an FITC filter. However I detect
the same flouescence in both transformed and untransformed cells.
Help. Any suggestions????
Julianne Smith, UMR 218
Institut Curie Sction de Recherche
26, rue d'Ulm
Paris 75231 Cedex 05
Phone: 01 42 34 66 90