Has anybody heard of a GFP variant (or other fluorescent protein) that has
an excitation maxima around 488nm, but emits in the yellow (580nm) or long
red wavelengths (670nm). The addition of the Blue variant of GFP is useful
as a second marker to GFP, but most of the common instrumentation is not
set up to examine the two excitation and emission wavelengths
simultaneously. A Yellow or Red fluorescent protein that is stably
expressed like EGFP and efficiently excited at 488nm would be more useful
as a second marker with our equipment.
This is probably like asking for the goose that laid the golden egg!
Damian F.J. Purcell, Ph.D.
Macfarlane Burnet Centre .***. .***. .**
PO Box 254 _--_|\ * | | | * * | |
Fairfield, VIC 3078 / \ * * | | | * * | | |
AUSTRALIA. \_.--._/ * * | | | * | | | *
ph. (+61 3) 9282 2256, O '***' '***'
FAX (+61 3) 9282 2100