There is a wonderful article that was published in the the journal of
microscopy several months ago (I'm sorry I can't remember the volume,
date, etc. but if you go to the www and search for clontech, they have a
section specifically for new gfp papers. the paper is quoted there and i
believe the author's last name is Niswender or something like that).
to quantify your samples, you might want to make a cell extract and use a
flurometer. also, someone mentioned to me that flow cytometry is another
method to quantify your gfp in culture.
i'll let you know if i come across any articles regarding "quantifying
gfp." in the meantime, good luck.
On 16 Oct 1997, R. Maney wrote:
>> I know this must be an often asked question but...
>> I was wondering if someone could clue me in on quantifying GFP expressed
> in tissue? All I need to know is relative amounts of GFP expressed.
> Can this even be done?
>> Todd Maney