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quantifying GFP

Neva Morales nmorales at ACSU.BUFFALO.EDU
Fri Oct 17 13:53:04 EST 1997

HI Todd,

There is a wonderful article that was published in the the journal of
microscopy several months ago (I'm sorry I can't remember the volume,
date, etc. but if you go to the www and search for clontech, they have a
section specifically for new gfp papers. the paper is quoted there and i
believe the author's last name is Niswender or something like that).

to quantify your samples, you might want to make a cell extract and use a
flurometer. also, someone mentioned to me that flow cytometry is another
method to quantify your gfp in culture.

i'll let you know if i come across any articles regarding "quantifying
gfp." in the meantime, good luck.


On 16 Oct 1997, R. Maney wrote:

> I know this must be an often asked question but...
> I was wondering if someone could clue me in on quantifying GFP expressed
> in tissue?  All I need to know is relative amounts of GFP expressed. 
> Can this even be done? 
> Thanks
> Todd Maney

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