> I know this must be an often asked question but...
>> I was wondering if someone could clue me in on quantifying GFP expressed
> in tissue? All I need to know is relative amounts of GFP expressed.
> Can this even be done?
If you are working with a cell culture, flow cytometry analysis is the best
way. The intensity of fluorescence will be proportional to the GFP
expression level, if you are using the EGFP variant.